From Ionic Currents to Molecular Mechanisms

From Ionic Currents to Molecular Mechanisms The Structure and Function of Voltage-Gated Sodium Channels

tetrodotoxin binding activity and a protein of ‫072ف‬ kDa (Agnew et al., 1980). Subsequent purification studies showed that the sodium channel from mammalian brain is a complex of ␣ al., 1982), the tetrodotoxin-binding component of the sodium channel purified from eel electroplax is a single Voltage-gated sodium channels are responsible for the protein of 270 kDa (Miller et al., 1983), and the ...

From Ionic Currents to Molecular Review Mechanisms: The Structure and Function of Voltage-Gated Sodium Channels

Cardiac ionic currents and acute ischemia: from channels to arrhythmias.

The aim of this review is to provide basic information on the electrophysiological changes during acute ischemia and reperfusion from the level of ion channels up to the level of multicellular preparations. After an introduction, section II provides a general description of the ion channels and electrogenic transporters present in the heart, more specifically in the plasma membrane, in intracel...

Ionic gradients, membrane potential and ionic currents

The neuronal plasma membrane delimits the whole neuron, cell body, dendrites, dendritic spines, axon and axon terminals. It is a barrier between the intracellular and extracellular environments. The general structure of the neuronal plasma membrane is similar to that of other plasma membranes. It is made up of proteins inserted in a lipid bilayer, forming as a whole a ‘fl uid mosaic’ ( Figure 3...

Ionic currents in single cells from human cystic artery.

The patch-clamp technique was used to study the electrophysiological properties of single smooth muscle cells obtained from the human cystic artery. These cells contracted on exposure to high K+ and had a mean resting potential of -36 +/- 7 mV. Under current clamp, regenerative responses could not be elicited when depolarizing pulses were applied. Voltage-clamp measurements demonstrated that a ...